By Dr. Pham Van Toan

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24, 525-533 5. , 1979. Assimilation and transport of nitrogenous compounds originated from 15N2 fixation and 15NO3 absorption, Soil Sci. , 25, 9-19 6. , 1980a. Nitorgen assimilation in soybean nodules I. The role of GS/GOGAT system in the assimilation of ammonia produced by N2-fixation, Soil Sci. , 26, 109-115 7. , 1980b. Nitorgen assimilation in soybean nodules II. 15N2 assimilation in bacteroid 39 and cytosol fractions of soybean nodules, Soil Sci. , 26, 205-213 8. , 1980c. Nitrogen assimilation in soybean nodules III.

Plant Cell Physiol. 15, 655-661 (1974) 5. F. Chemical and isotopic studies of the nitrox system for N15 enrichment. ) 6. OHYAMA, T. 1982. Emission spectrometric 15 N analysis of amino acids. ) RADIOISOTOPES, 31, 212-221 (in Japanese) 7. , KOBAYASHI, K. , and IKARASHI, T. 1991. Analytical procedures of N, P, K contents in plant and manure materials using H2SO4 –H2O2 Kjeldahl digestion method. Bull. Facul. Agric. , 43, 111-120 (in Japanese) 8. NISHIWAKI, T. , and OHYAMA, T. 1994. Determination of nitrate content in soybean by Cataldo’s method.

We make tubes under continuous vacuum conditions without closing the system. After evacuation of the air inside the tubes, the part of the outside tubes up to about 2cm-10 cm from the end are heated gently by oxygen gas burner from down up direction to remove the adsorbed water in the glass wall. About 2 cm long from the bottom of the Pyrex tube should not be heated, because sample N will be converted to N2 and lost. After the pressure inside the discharge tubes reaches below 10-3 Torr (mg Hg), the tubes are cut off and sealed by oxygen gas burner, for about 4 cm (short tube for low N) to 8 cm (long tube for high N) from the bottom (Fig.

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