By M. Yamashita (auth.), K. Nagai, M. Wachi (eds.)

Animal telephone know-how is a transforming into self-discipline of phone biology which goals not just to appreciate constructions, services and behaviors of differentiated animal cells but additionally to discover their skills for business and clinical reasons. The aim of animal telephone know-how comprises clonal growth of differentiated cells with helpful skills, optimization in their tradition stipulations at the business scale, modulation in their skill so as successfully to supply medically and pharmaceutically vital proteins, and alertness of animal cells to gene treatment and formation of man-made organs. This quantity supplies the readers an entire evaluation of the current state-of-the-art in Japan, a rustic the place this box is easily complex, in addition to in Asia, Europe and the us. The lawsuits could be valuable for mobilephone biologists, biochemists, molecular biologists, biochemical engineers and people in different disciplines on the topic of animal cellphone tradition, operating in educational environments in addition to within the biotechnology and pharmaceutical industries.

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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Ninth Annual Meeting of the Japanese Association for Animal Cell Technology, Yokohama, Japan, September 1–4, 1996

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Japan. Abstract NAT-30 (U,2) is a human plasma cell line derived from Namalwa, a human Burkitt lymphoma cell line (1). It was previously demonstrated that concanavalin A (Con A) stimulation of the NAT-30 cells caused the loss of the original light chain of the NAT-30 cells and the expression of the new light chains in this cell line (2). This phenomenon was referred to as Light Chain Shifting (2).

Culture with 3 pM EPO (approx. 100 pg/ml= 10 munits/ml) increased the number of viable cells significantly, andculture with 30 pM EPO almost completely protected neurons from glutamate toxicity. The coexistence of the soluble EPOR over EPO abrogated almost completely the protective effect of EPO. U B ::; 20 0 Glu EPO(pM) 0 + 0 + 3 + 30 + 300 + 300 +sEPOR Figure 3. (/eft) Immunochemical detection of EPOR in cultured hippocampal neurons. A Cells were stained with the anti-EIDR antibody. B Cells were stained with the antibody preabsorbed with the antigen.

The cells were cultured with changes of the medium every 3 days and EFD in the spent medium was measured. d. of sextuplicate cultures. A, the cells were cultured in 21 % (0) and 5% (e) of 02' B, oxygen tension was changed to 1, 5, and 21 % of 3days intervals. C, oxygen tension was changed to 21,5, and 1% in this order. 27 25 IGFs. EPO production was stimulated by IGFs or insulin in a dose-dependent manner (Fig. 2A). Half maximal stimulation of EPO production was achievedby 3 nM IGF-I,lO nM IGF-II and 100 nM insulin.

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